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| Registros recuperados: 44 | |
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| Le Guillard, Cecile; Dumay, Justine; Donnay-moreno, Claire; Bruzac, Sandrine; Ragon, Jean-yves; Fleurence, Joel; Berge, Jean-pascal. |
| The aim of this study was to compare two processes for the extraction of R-phycoerythrin (R-PE) from the red seaweed Grateloupia turuturu: ultrasound-assisted extraction (UAE) and ultrasound-assisted enzymatic hydrolysis (UAEH). Process efficiencies were both evaluated by the yield of R-PE extraction and by the level of liquefaction. Experiments were conducted at 40 and 22 °C, for 6 h, using an enzymatic cocktail and an original ultrasonic flow-through reactor. R-PE appeared very sensitive to temperature, thus 22 °C is strongly recommended for its extraction by UAEH or UAE. However, the higher processing temperature (40 °C) clearly increased the extraction of water-soluble compounds (up to 91% of liquefaction). These two new processes are thus promising... |
| Tipo: Text |
Palavras-chave: Seaweed; Grateloupia turuturu; R-phycoerythrin; Ultrasound-assisted extraction; Ultrasound-assisted enzymatic hydrolysis; Liquefaction. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00292/40332/39993.pdf |
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| Perez-galvez, Raul; Berge, Jean-pascal. |
| Introduction : A direct consequence of traditional fishing practices is the considerable quantities of wastes and byproducts that are generated. They represent an economical and environmental problem since not all obtained from the sea is adequately used but thrown back into the sea as a waste. With the aim of classifying and quantifying the by-products, three main groups are considered: discards, wastage on board and by-products and wastage on shore. |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7409.PDF |
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| Perez-galvez, Raul; Guadix, Emilia M.; Berge, Jean-pascal; Guadix, Antonio. |
| BACKGROUND The press juices resulting from a compacting operation on fish by-products were subjected to a depuration treatment in order to reduce the high COD (120 g O2 L–1). The process included an initial de-particulation step by means of two metallic filter cartridges of 465 µm and 250 µm, followed by concentration with a 200 nm ultrafiltration ceramic membrane. The polishing efficiency of each unit was assessed in terms of COD and protein removal. RESULTS Dead-end metallic filtration of the press waters reduced their suspended matter content by 28%, but achieved only 5.6% decrease in chemical oxygen demand (COD), which suggested further processing by membrane ultrafiltration. The de-particulated stream was then subjected to ultrafiltration for 8 h in... |
| Tipo: Text |
Palavras-chave: Membranes; Waste-water; Bioseparations; Filtration. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00156/26701/24791.pdf |
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| Olofsson, Martin; Lamela, Teresa; Nilsson, Emmelie; Berge, Jean-pascal; Del Pino, Victoria; Uronen, Pauliina; Legrand, Catherine. |
| While focus in oil-producing microalgae is normally on nutrient deficiency, we addressed the seasonal variations of lipid content and composition in large-scale cultivation. Lipid content, fatty acid profiles and mono- di- and triglycerides (MAGs, DAGs, and TAGs) were analyzed during May 2007-May 2009 in Nannochloropsis oculata grown outdoors in closed vertical flat panels photobioreactors. Total lipids (TL) ranged from 11% of dry weight (DW) in winter to 30% of DW in autumn. 50% of the variation in TL could be explained by light and temperature. As the highest lipid content was recorded during autumn indicating an optimal, non-linear, response to light and temperature we hypothesize that enhanced thylakoid stacking under reduced light conditions resulted... |
| Tipo: Text |
Palavras-chave: Biodiesel; Microalgae; Seasonal variation; Nannochloropsis oculata; Large-scale; Lipids; Fatty acids; Light; Temperature. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00085/19582/17206.pdf |
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| Cardinal, Mireille; Cornet, Josiane; Donnay-moreno, Claire; Gouygou, Jean-paul; Berge, Jean-pascal; Rocha, E.; Soares, S.; Escorcio, C.; Borges, P.; Valente, L. M. P.. |
| This study describes the variability of important quality traits in gilthead sea bream produced under intensive conditions in Southern Europe and purchased in a French market (Nantes). Chemical, sensory and histological characteristics were performed on 18 samples delivered over a year. All fish presented far lower dioxin and DL-PCBs or heavy metals concentrations than the maximum limit level set by EC Regulation, being totally safe for human consumption. Fat content and nutritional values reflected by n-3 and n-6 polyunsaturated fatty acids (PUFA) levels were parameters with high variability according to the season or the fish farm. Values of n-3 PUFA ranged from 0.9% to 8.6% and those of n-6 PUFA from 9.3% to 16.6%, corresponding to a respective... |
| Tipo: Text |
Palavras-chave: Quality parameters; Sea bream; Sparus aurata; Farmed fish; Chemical composition; Fatty acids; Sensory evaluation; Histological characteristics. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00016/12757/9691.pdf |
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| Bourseau, Patrick; Vandanjon, Laurent; Jaouen, Pascal; Chaplain-derouiniot, Maryse; Masse, Anthony; Guerard, Fabienne; Chabeaud, Aurélie; Fouchereau-peron, Martine; Le Gal, Yves; Ravallec-ple, Rosenn; Berge, Jean-pascal; Picot, Laurent; Piot, Jean -marie; Batista, Ireneu; Thorkelsson, Gudjon; Delannoy, Charles; Jakobsen, Greta; Johansson, Inger. |
| The production by enzymatic treatment of fish protein hydrolysates (FPH) is a promising route to add value to fisheries proteinic co-products (fish frames, heads etc.). Indeed, FPH possess good nutritional properties and biological activities for food and feed uses. Pressure-driven membrane separations such as ultrafiltration (UF) and nanofiltration (NF) can be used after the hydrolysis to, increase the specific activities of the FPH. This paper discusses the impact of a two-step UF/NF process producing four different fractions on two industrial FPH with different hydrolysis degrees. Fractionation is carried out in "realistic" conditions for an industrial process, on highly concentrated FPH solutions (about 100 g of dry matter/L) at a high volume reduction... |
| Tipo: Text |
Palavras-chave: Peptidic profile; Fractionation process; Membrane separation; Nanofiltration; Ultrafiltration; FPH; Fish protein hydrolysate. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6653.pdf |
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| Le Nechet, Sandrine; Dubois, Nolwenn; Gouygou, Jean-paul; Berge, Jean-pascal. |
| If rays are traditionally fished for their caudal fins, the rest of the body is wasted, except part of the skin that can be transformed into leather. Liver oil of the ray, Himantura bleekeri, was characterized in terms of lipid class composition and fatty acid profile. Liver oil content was high and represented 54% of the liver weight (w/w). Neutral lipids were predominant (92%), major components being triacylglycerols (63%). Other neutral lipids identified were hydrocarbons, sterol esters, mono- and di-acylglycerols, free fatty acids, sterols and glyceryl ethers. Polyunsaturated fatty acids of the n-3 series, namely eicosapentaenoic and docosahexaenoic acids, were high (4% and 16%, respectively). Cholesterol was the major component in the sterol fractions... |
| Tipo: Text |
Palavras-chave: Sterol; Squalene; DHA; EPA; PUFA; Lipids; Fatty acids; Ray liver oil. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3129.pdf |
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| Dubois, Nolwenn; Barthomeuf, Chantal; Berge, Jean-pascal. |
| It is essential to have simple rapid methods for the determination of fatty acid structures. Traditionally, fatty acids are analysed by gas chromatography using their methyl ester derivatives (FAME). However, their corresponding mass spectra exhibit molecular ions but are usually devoid of ions indicative of structural features and, notably, the position of double bounds on the aliphatic chains [1]. In the most useful approach to structure determination, the carboxyl group is derivatised with a reagent containing a nitrogen atom. Recently, a convenient method for preparing picolinyl esters from intact lipids has been published [2]. However, some problems occurred in our laboratory when this method was used, leading to some modifications and optimisation.... |
| Tipo: Text |
Palavras-chave: Sensitivity; Sterol esters; Fatty acid methyl esters; Gas chromatography; Mass spectrometry; Picolinyl esters. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2125.pdf |
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| Nguyen, Thi-my-huong; Perez-galvez, Raul; Berge, Jean-pascal. |
| A feeding trial was conducted to evaluate the effect of the supplementation of hydrolysates from tuna head on the survival and growth of Penaeus vannamei. To this end, a feeding trial was performed on eleven groups of shrimps of average weight 4.34 g. Every group was distributed into three tanks (30 shrimps per tank of 150 L) and was fed with one of the following eleven diets, all of them containing 40% crude protein: one diet containing tuna head meal as the principal protein source, which was used as control diet; nine diets formulated by replacing 50% tuna head meal by fish protein hydrolysates (FPH), and finally one commercial diet chosen as a reference. FPH were obtained by hydrolysis of tuna head during 2, 3 or 6 h and ulterior centrifugation in order... |
| Tipo: Text |
Palavras-chave: Tuna; Head; Fish protein hydrolysates; Growth of shrimp. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00064/17517/15138.pdf |
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| Perez-galvez, Raul; Guadix, Emilia M.; Berge, Jean-pascal; Guadix, Antonio. |
| This paper assesses the performance of three ceramic membranes of average pore size 50 nm, 200 nm and 1.4 tan in the treatment of the press liquor resulting from a compaction operation of sardine by-products. To this end, 2 L of pre-filtered press liquor with an average protein content of 44 g/L were filtered during 3 h through the three membranes assayed. Unexpectedly, the membrane of larger pore size, presented the lowest values for the flux of permeate at steady state at 20 L/(m(2) h), while its average protein rejection (77%) was similar to that observed in the membrane of 200 nm. This fact was attributed to the difference in material of its active layer (a-alumina instead of zirconite). The cleaning efficiency of the membranes was assessed by the COD... |
| Tipo: Text |
Palavras-chave: Fish processing; Ceramic membranes; Ultrafiltration; Fouling; Cleaning. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00052/16290/14084.pdf |
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| Soufi-kechaou, Emna; Jaouen, Pascal; Ben Amar, Raja; Berge, Jean-pascal. |
| Enzymatic hydrolysis of cuttlefish viscera by Protamex, Pepsin, Delvolase and Multifect Neutral was investigated in a batch reactor during 6 hours with an hourly sampling. The influence of the enzyme used and the hydrolysis conditions (operating pH, temperature) was studied with regards to the extent of proteolytic degradation and to molecular weight distribution of the peptides. After 360 min of hydrolysis, cuttlefish viscera treated with Pepsin yielded significantly (p < 0.05) higher protein recoveries compared to the substrate autolysed or treated with other external proteases. However, the amino acid composition showed that this was Protamex hydrolysis which gave the highest percentage of essential amino acids. At least, according to the FPLC... |
| Tipo: Text |
Palavras-chave: Enzymatic hydrolysis; Cuttlefish Protein Hydrolysates (CPH); Cuttlefish By-Products (CBP); Essential Amino Acids (EAA); Protein recovery. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00114/22508/20191.pdf |
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| Nguyen, Thi-my-huong; Sylla, Khalifa Serigne Babacar; Randriamahatody, Zo; Donnay-moreno, Claire; Moreau, Jacques; Luyen Thi Tran,; Berge, Jean-pascal. |
| Long-term proteolysis of tuna by-products (head, viscera and tail) by the wide spectrum protease Protamex has been investigated and compared. After hydrolysis, two fractions (soluble aqueous phase and insoluble sludge) were collected. Chemical compositions of each fraction and molecular mass distributions of soluble peptides were determined. Degrees of hydrolysis obtained after 12 h of hydrolysis of head, viscera and tail were 32.3, 16.8 and 22.2 %, respectively. Nitrogen recovery in the soluble fractions was 73.6 % for the head, 82.7 % for the viscera and 85.8 % for the tail. Lipid distribution indicated that the majority of lipids remained in the sludge. Such proteolysis appears useful for the production of very different fractions: one rich in peptides... |
| Tipo: Text |
Palavras-chave: Tuna; By-products; Proteolysis; Nitrogen recovery; Fish protein hydrolysates. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00030/14142/14042.pdf |
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| Besbes, Nadia; Jerome, Marc; Berge, Jean-pascal; Sadok, Saloua. |
| This study describes a polymerase chain reaction using restriction fragment length polymorphism (PCR-RFLP) assay based on the 16S rRNA mitochondrial gene to identify commercial food products of wide range of Penaeidae and Pandalidae shrimp species commercialised in the Tunisian market. Phylogenetic analyses on 16S rRNA mitochondrial gene were used to study the relationships among the considered species. Penaeidae shrimp species was easily differentiated and confirmed by direct sequencing, showing a genetic distance of 0.34 with respect to Pandalidae species. A rapid and reliable PCR method using restriction fragment length polymorphism (RFLP) with three restriction enzymes (HpyCH4III/ MboI / AluI) was optimized for unambiguous differentiation of shrimp... |
| Tipo: Text |
Palavras-chave: Shrimps; Species identification; 16S rRNA mtDNA; PCR-RFLP. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00303/41445/40637.pdf |
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| Thorkelsson, G; Sigurgisladottir, S; Geirsdottir, M; Jóhannsson, R; Guerard, Fabienne; Chabeaud, Aurelie; Bourseau, Patrick; Vandanjon, Laurent; Jaouen, Pascal; Chaplain-derouiniot, M; Fouchereau-peron, Martine; Martinez Alvarez, Oscar; Le Gal, Yves; Ravallec-ple, Rozenn; Picot, Laurent; Berge, Jean-pascal; Delannoy, Charles; Jakobsen, Greta; Johansson, I; Batista, Ireneu. |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00056/16747/14184.pdf |
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| Olofsson, Martin; Lamela, Teresa; Nilsson, Emmelie; Berge, Jean-pascal; Del Pino, Victoria; Uronen, Pauliina; Legrand, Catherine. |
| Instead of sole nutrient starvation to boost algal lipid production, we addressed nutrient limitation at two different seasons (autumn and spring) during outdoor cultivation in flat panel photobioreactors. Lipid accumulation, biomass and lipid productivity and changes in fatty acid composition of Nannochloropsis oculata were investigated under nitrogen (N) limitation (nitrate:phosphate N:P 5, N:P 2.5 molar ratio). N. oculata was able to maintain a high biomass productivity under N-limitation compared to N-sufficiency (N:P 20) at both seasons, which in spring resulted in nearly double lipid productivity under N-limited conditions (0.21 g L−1 day−1) compared to N-sufficiency (0.11 g L−1 day−1). Saturated and monounsaturated fatty acids increased from 76% to... |
| Tipo: Text |
Palavras-chave: Microalgae; Outdoor; Nannochloropsis; Large-scale; Lipids; Fatty acids; Nitrogen manipulation; Seasonal changes; Biofuels; High value products. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00184/29564/27902.pdf |
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| Hardouin, Kevin; Bedoux, Gilles; Burlot, Anne-sophie; Donnay-moreno, Claire; Berge, Jean-pascal; Nyvall-collen, Pi; Bourgougnon, Nathalie. |
| In order to develop innovative methods for the production of bioactive extracts with potential health benefits, enzyme-assisted extraction has been applied for the bioconversion of Ulva armoricana (Ulvales, Ulvophyceae). The extraction yields, chemical composition, the antioxidant and antiviral activities were determined to assess the efficiency of six commercial enzymatic preparations. Endo-protease treatments significantly increased the extraction yields compared to the control. The organic matter, neutral sugar and protein contents were increased in all extracts compared to an extraction with water, up to 2.0-fold, 2.7-fold and 1.75-fold, respectively. The samples prepared by a multiple-mix of glycosyl-hydrolases and an exo-β-1,3(4)-glucanase revealed... |
| Tipo: Text |
Palavras-chave: Ulvan; Antiviral activity; Carbohydrate; Endo-protease; Bioconversion; Biorefinery. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00321/43187/45542.pdf |
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| Registros recuperados: 44 | |
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